Produce a Michaelis-Menten plot showing enzyme velocity (V0) vs [S].

Description

Using the table provided, please supply each of the dot points below with correct titles, values and descriptions. – Use the initial velocities and your standard curve to determine the rate that pNP was produced at each substrate concentration (this can be considered enzyme velocity shown as [pNP]/time). That is, convert to [pNP]/time from Absorbance/time. – Produce a Michaelis-Menten plot showing enzyme velocity (V0) vs [S]. You may be able to estimate Km and Vmax from this. – Prepare a table of reciprocal V0 and [S] to be used for a Line-weaver Burk plot. Note that reciprocal V0 and [s] simply means 1/V0 and 1/[S]. – Produce a Line-weaver Burk plot (ensure you label each axis correctly). You will need to extend the trendline so that your graph shows where the trendline intercepts the x-axis and y-axis. – Use the Line-weaver Burk plot to determine Km and Vmax. You can use the equation of the line to first determine the -1/Km and 1/Vmax and then rearrange to find Km and Vmax.