Explain errors and/or inconsistencies that might have affected the data. Data can naturally vary due to changes in conditions. Human error should not be included in the discussion of changes in data. Human error is not considered a root cause affecting data, but as a flaw in using the best practice when carrying out the experiment. Think of other things that might have affected your results or possible limitations of the experiment if your data looks good.

Must Include:
1. Introduction
A. Give enzyme background (where, what, how? for example, where does this reaction take place, what are enzymes, etc.).
B. Write out the catecholase reaction in equation format.
C. Explain variable being tested. What are the reactants? What is the substrate?
D. Discuss and explain catecholase and benzoquinone.
E. Discuss chelating agents.
F. Include observations leading to clear statement of hypothesis.
G. Include the independent and dependent variables.
3. Materials and Methods MUST ALSO INCLUDE POTATO PREP(shown in attachments)
A. Use the proper names of all materials and explain all materials used.
B. Make data collection protocol clear (explain what you did).
C. Justify methodology (explain how and why you did each step in enough detail so that someone else can repeat your experiment).
D. State control clearly and explain how it is a control and why is it used as a control. What do you expect to see in the control?
E. Explain relationship between color intensity and amount of reaction.
F.
Include how and why we calculate a change in absorbance.
4. Results
A.
Summarize your results.
i. Characterize and describe trends for independent variables. Make sure to include absorbance values.
5. Discussion
A.
Support or reject hypothesis and give rationale for this claim.
i. State correct cofactor (as determined by your data) and explain how this was deter-mined. Refer to the data by treatment, not Tube #.
B.
Relate control to interpretation of results.
C. Explain errors and/or inconsistencies that might have affected the data. Data can naturally vary due to changes in conditions. Human error should not be included in the discussion of changes in data. Human error is not considered a root cause affecting data, but as a flaw in using the best practice when carrying out the experiment. Think of other things that might have affected your results or possible limitations of the experiment if your data looks good.
D. Explain relationship of factor tested to amount of enzyme activity.
E. Place results in the bigger picture. Why should we care about these results?
6. Appendix
A. Include all relevant data.
B. Label graphs, tables, and figures properly. Use a descriiptive title.
C. Provide reference graphs and tables in text of results section.
D. Use the appropriate type of graph to display the data.
7. Literature Cited
A. Include the lab manual. All references other than the lab manual (and textbook if used) must have in-text citations as well.
B. Reference materials within report by paraphrasing (written in your own words).