Discuss specific problems you had with the procedure and how these errors could have influenced your results.

Assignment – Yeast Cloning and Sequencing Project Lab Report This report is the full lab report you are required to write for this course and is worth 8% of your final course grade. The book “A Short Guide to Writing about Biology” by Jan A. Pechenik is an excellent reference for scientific writing. If you do not have this book, you may access a copy in the lab or library. The current edition of Pechenik is on reserve in the library and older editions can be found in the stacks. We have provided a pdf copy of Chapter 9 (pp. 149-220) for your reference. Basic Guidelines for Lab Report • Your full lab report will include a Title page, Abstract, Introduction, Materials and Methods, Results, Discussion, References, and Appendix sections. • Your report will be a maximum of 10 pages of typed text (excluding the title page, embedded figures and tables, references, and appendix). • Your report will be double spaced, 12-point font, printed one-sided on 8.5 x 11 printer paper and stapled at the top left corner. • Your report MUST be written individually and contain no Academic Integrity Violations. • Reports will be written using APA 7 th edition. Information on the new APA style can be found here: https://libguides.kpu.ca/c.php?g=713337&p=5083418. • Cite other people’s ideas (e.g. other researchers, textbook authors, information in peer reviewed articles, etc.) using APA style in-text citations, and include a minimum of two credible references, other than the lab manual and the required course text. Blogs, Wikipedia pages, or personal websites are not credible sources. Peer reviewed science articles, journal articles, and textbooks are credible sources. Information on university websites, official government websites (e.g. www.genome.gov) or large science organizations (e.g. www.nature.com/scitable/) may be acceptable (review the about page and ask a librarian when in doubt). When considering whether or not a website is acceptable, ask yourself if it is peer reviewed by experts in the field (e.g. Wikipedia is peer-reviewed, but NOT necessarily by experts in the field, as anyone can submit edits, which is why it is not an acceptable source). • Along with content you will be marked on correct spelling, grammar and formatting, clear and concise writing, following scientific conventions, etc. • Include any raw data, rough calculations, and anything else essential to the report in the appendix. Organization of ideas Each section of your lab report serves a particular purpose. While writing your lab report it is important that you put information in the correct section of your report, and that the information within a section is well organized and flows in a logical order. The information provided in the following pages is intended to be used as a guide to help you place information in the correct section of your report. Your role, while writing and editing these sections, is to organize and communicate the information in a way that will make sense to your reader. Yeast Cloning and Sequencing Project Title page Provide a title page in APA format. Instructions on how to make a title page can be found here: https://apastyle.apa.org/style-grammarguidelines/paper-format/title-page. Abstract This section should include:  A brief introduction to the lab, brief summaries of methods, major results and interpretation of findings.  The summary of the results should include: (a) if you successfully obtained a sequence, (b) the name of gene identified (if applicable) or indicate that you found a non-coding sequence, (c) E-value of sequence alignment, (d) description of gene function (if applicable), and (e) how your findings relate to those of other researchers. This single paragraph includes only the most relevant information and should be 1/3 to 1/2 of a page in length. Introduction This section should include:  A discussion of yeast (saccharomyces cerevisiae) as a model organism including a description, reasons for its use in this project, and reasons for its use in genetic studies.  A discussion of the role of bacteria and specifically why JM101 E. coli cells were used.  A discussion of the role of the pUC19 plasmid and why it was used.  A description of the blue-white screening system.  A clear and concise statement of the purpose of the project.  In-text citations (use 7th edition APA citation style). The content in this section should be clear, concise, well organized and flow in a logical order (i.e. the information does not need to be given in the order listed above). Materials & Methods This section should include:  A reference to the procedure in the lab manual. o Start this section with a general statement such as “The procedures in this experiment were performed as described in the Yeast Cloning and Sequencing Project Parts 1, 2, 3 & 6 (followed by an in-text citation for the lab manual).” o We are giving you permission to use this language, so it will not be considered plagiarism if you use it, however the rest of this section needs to be in your own words.  A brief description of each step in the project. This section of your report will be 6-8 paragraphs in length (1 ½ to 2 ½ pages), depending on how you divide up the experiment and organize the information.  A description of any deviations from the procedure and their rationale (deviations should be included in the summary of the procedure and not added to the end of the report).  A description and discussion of the purpose of all controls used throughout the project. Revised Jan. 26th, 2022 The content in this section should be clear, concise, well organized and flow in a logical order. It should be written in the past tense as the experiment has already been performed. It should include the appropriate level of detail (i.e. provide an overview of the most important parts of the experiment, but not list every single procedural step). Results This section should include: • A written description of (a) the results of the controls, (b) experimental results (e.g. results of the platings, whether or not you obtained a gene sequence, transformation efficiency value, etc.), (c) summary of the results of the BLAST analysis in one to two sentences (e.g. name of gene(s) and/or presence of a non-coding region; E-value, brief summary of gene function, etc.). • Data should be presented as tables and figures and embedded in the written results section where appropriate. Raw data goes in the Appendix. Include the following tables and figures: o A table of transformation results including the controls. o The sequence of the yeast DNA fragment cloned (presented as a figure). • All figures and tables should be appropriately numbered and have detailed captions. Refer to all tables and figures in the written text. E.g. (see Figure 1). Do not interpret the results in this section. Interpretation of findings go in the Discussion. Discussion This section should include: • A discussion of what you cloned (either part of a gene(s) and/or a non-coding sequence) including the likelihood of each result (i.e. how likely are you to clone a gene vs. a noncoding region?). • A discussion of the confidence in sequence identification (E-value) including an explanation of what an E-value is and what your specific E-value means. • If the sequence was part of a gene, discuss the gene’s function(s). • If the sequence was non-coding, indicate if it was intronic or intergenic sequence. • A discussion of how your results compare to that of other researcher (if possible). In other words, describe what else in known about this gene. • Suggestions for future experiments related to the findings of this experiment (hint: you can base your future experiments on your research regarding gene function). • Discuss specific problems you had with the procedure and how these errors could have influenced your results. • A concluding statement. • In-text citations where appropriate (APA citation style). Appendix This section, which will be located at the back of your lab report, should include: • a copy (printed screen-shot) of your E-value page (from the BLAST search), • a copy (printed screen-shot) of the Genome Browser summary page, • transformation efficiency calculations (can be typed or handwritten). Yeast Cloning and Sequencing Project Revised Sept. 3rd, 2021 For more information on how to write each section of a lab report refer to the Appendix of the lab manual and Pechenik. Example of APA Citation Style. In-text citation: Below is a fictitious example of a paraphrased sentence from the Introduction section of a student’s lab report. It gives credit to the original source (this is the first time the source is being cited). The pUC19 plasmid is an ideal plasmid for a transformation experiment because it contains the lacZ gene which can be used for blue-white screening of recombinant molecules (National Center for Biotechnology Information [NCBI], 2020). Note if you reference this source again, subsequent references can be abbreviated to (NCBI, 2020). If you are citing multiple sources with the same author and date, then include a lowercase letter after the year in the intext citation and corresponding reference (i.e. in-text citation would be (NCBI, 2020a) or (NCBI, 2020b)).

Consider to what extent is the UK’s new points-based visa system consistent or inconsistent with the International Convention on Migrant Workers.

Immigration and Refugee Law

Coursework assignment

Critique 4,000 words

Choose one of the following topics and write critical essay of 4,000 words. The word count excludes the bibliography and footnote references.

1. Consider to what extent is the UK’s new points-based visa system consistent or inconsistent with the International Convention on Migrant Workers.

2. Critically analyse the UK Supreme Court’s decision in Begum v Secretary of State for the Home Department [2021] UKSC 7 from the point of international law on citizenship and statelessness.

In your critique answer you need to make explicit reference to relevant international and regional agreements, customary international law, case law of the relevant courts and/or bodies as well as academic books and peer-reviewed academic journal articles accessed principally through the University Library. You should conduct your own research and use the chapters in the recommended textbooks for this module as your starting point.

Please reference your work using the OSCOLA reference system and include a full bibliography of all sources you have used at the end of your work.

Module learning outcomes (LOs)

1 – Evaluate the various sources, institutions and procedures in the field of international migration and asylum law.

2 – Critically appraise the theoretical debates in the field and engage in critical legal analysis of the practice of judicial and other institutions.

3 – Critically reflect on the role of the law in shaping the experiences of migrants and asylum seekers, including the protection of their rights.

4 – Carry out independent research in the field of international migration and asylum law and policy using both library-based and electronic resources.

General Assessment Criteria (D)

KNOWLEDGE:

1. Clear statement of relevant law
2. Excellent statement of relevant law

APPLICATION:

1. Obvious and subtle issues dealt with, good application of law
2. Excellent coverage of issues, excellent application of law

EVALUATION:

1. Good evaluation, critical judgment, and problem solving
2. Excellent evaluation, critical judgment, and problem solving

RESEARCH:

1. Clear referencing, evidence of some detailed research
2. Excellent footnote referencing, bibliography, and research

COMMUNICATION:

1. Good spelling and grammar, well organised structure
2. Excellent spelling, grammar, structure, and presentation

Write an abstract for the presentation to attract an audience of non- specialists to watch your presentation.

Contemporary Global Issues 3: Abstract

Task:                          Write an abstract for the presentation that you will be giving in week

12. The purpose of the abstract is to attract an audience of non-

specialists to watch your presentation.

Word Length:                       250 words

Instructions:            The 4 elements required in an abstract should be explicit in your text:

1. Background to the issue (why is this issue important or interesting?)
2. Research/methodology (what research did you do in order to find out about this issue?)
3. Results (what did you learn about this issue from your research?)
4. Conclusions/implications (how significant is this research?)

You will need to state the aim of your presentation clearly.

You do not need to include in-text references (citations) or quotes, but a reference list is required at the end so that your tutor knows which texts you have read. You should use a minimum of 3 sources.

The list of references for the abstract should be the same as those for your presentation, apart from any which refer to pictures/graphs/charts which you used in your presentation.

Reflection (submit one reflection to cover both the presentation and abstract, but this will be 10% of the presentation mark only)

There is no specified word limit for the reflection task, (but approximately 500 words). On completion of your abstract AND presentation, you should write a reflection on how easy/difficult you found it, (ie. What did you think were your strengths and weaknesses?) which skills you feel were tested, and what you think you need to work on to make this type of task easier in future. You should submit this within 24 hours of completing the presentation via the Turnitin tool in the Assessment area of Minerva.

This abstract is worth 10% of your mark for this module

Please refer to the assessment criteria on Minerva.

Submission Instructions:

You should complete and attach a Cover Sheet to the front of your Abstract, which is on Minerva in the ‘Assessment’ area.

You must submit an electronic copy of your essay via Turnitin in the ‘Submit my work’ tab of the ELU0036 area on Minerva.

You should submit one reflection covering both the abstract and presentation tasks via Turnitin in the ‘Submit my work’ area of Minerva within 24 hours of giving your presentation.

Give an oral presentation on an interesting issue or recent development in your subject area to a non-specialist audience.

Contemporary Global Issues 3: Oral Presentation

Task:              Give an oral presentation on an interesting issue or recent development in your subject area to a non-specialist audience.

Timing: Your oral presentation must last for 7-8 minutes followed by 2-3 minutes for

questions.

Instructions: Your presentation must be related to a recent development or issue that you have chosen and researched independently. You must also be clear in your

introduction about whether your issue is:

1. an application of this subject to everyday life
2. a new development
3. c. a problem people are trying to solve
4. d. a controversial issue.

You must agree on the content you are proposing for the presentation with your tutor.

The content of your presentation must match the abstract you are submitting.

You will need to state the aim of your presentation clearly. You are going to present this topic/issue to non-specialists so it should not be anything too in-depth or technical.

You must include a slide with a list of references at the end of your presentation.

Your presentation will be recorded for assessment and moderation purposes.

You must submit your PowerPoint Slides (pdf) to Turnitin on the day of your presentation. Please ensure that your full name and student ID number are on your first slide.

Reflection (submit one reflection to cover both the presentation and abstract):

There is no specified word limit for the reflection task. On completion of your abstract AND presentation, you should write a reflection on how easy/difficult you found it, (ie. What did you think were your strengths and weaknesses?) which skills you feel were tested, and what you think you need to work on to make this type of task easier in future. You should submit this within 24 hours of completing the presentation via the Turnitin tool in the Assessment/Submit my work area of Minerva.

This presentation with reflection is worth 20% of your mark for this module

Please refer to the assessment criteria on Minerva

Guidelines:

• In your introduction don’t forget to introduce yourself, explain which degree programme you are studying next year (with a very brief definition if you think it is needed. For example, what does a degree in ‘medical chemistry’ cover? Also mention what type of issue you are focusing on – a new development, a controversial issue, a problem to be solved, an application of your degree subject to everyday life, or perhaps it is a combination of these.

• The marking criteria ask for ‘excellent coverage & balance in the presentation’, which means that you need to look critically at your ‘issue’ and try to analyse rather than just describe, wherever possible. Are there any drawbacks to the new development you have chosen, for example? How might a new development compare to the older development it is replacing? If you are looking at a ‘controversial issue’, are you giving a balanced viewpoint supported by evidence so that you don’t alienate your audience?

• Make sure you give evidence to support what you are saying and examples to illustrate (‘examples / statistics presented to support ideas’) Remember that your audience don’t know the topic as well as you do and therefore it needs to be explained clearly and with reference to helpful examples. Do not overload with too much subject-specific vocabulary, and explain any terms that you think the audience won’t know (‘the content is appropriate for a non-specialist audience’).

• Finally, try to choose something you are interested in so that this interest is communicated to the audience. Try to get the audience on your side from the start with a hook where it might be a good idea to try and link your topic to something the audience might relate to (‘attempt to interest the audience and to gain and establish rapport’).

• Remember that the list of references for the presentation and abstract should be the same, apart from any which refer to pictures/graphs/charts which you use in your presentation.

Why is it significant and included in the system

Description Each student is expected to submit resource recreation abstracts. These abstracts are “snapshots” of a National Park Service managed area that is NOT a National Park. Each student will do one abstract selected from the following categories. Subsequent abstract are to be on different categories. 1. National Monuments, 2. National Battlefields, Battlefield Parks or Battlefield Sites, 3. National Recreation Areas, Seashores or Lakeshores, 4. National Historic Sites, Parks or Memorials, 5. National Scenic River, Trail or Wild and Scenic River. The abstract should include at least the following: 1. A description of the site, 2. Why is it significant and included in the system, 3. Location, 4. Why anyone would visit, 5. Why you selected this particular site.

Explore the representation of the victim (Tom Robinson) in To Kill a Mockingbird by Harper Lee.

Description

Explore the representation of the victim (Tom Robinson) in To Kill a Mockingbird by Harper Lee. The paper must explore the victimization of Tom Robinson and how he becomes a victim of the miscarriage of Justice. Tom Robinson is wrongfully convicted for the rape of Mayella Ewell and becomes a victim of the miscarriage of justice. It can be argued that Robinson was born a victim as he is discriminated by society. The essay must explore the literary techniques used in To Kill a Mockingbird to construct Tom Robinson’s case as a miscarriage of justice. The female narrative and gender roles of the 19th Century should also be discussed as well as the exploration of The Civil Rights Movement and how this had an impact on Robinson’s case. Furthermore, race and segregation need to be explored in this essay and the injustice taking place inside and outside the courtroom. The paper also needs to be referenced using the Harvard referencing style and include academic secondary sources from websites such as Jstor

Identify access and critically evaluate/review the relevant data and literature in systematic manner.

Module Reading and Resources List: https://bradford.rl.talis.com/modules/sow6012-d.html This handbook gives you valuable information about your module, including which topics you will be studying, how you will be assessed and what learning materials you will need. If you have any questions after reading this handbook or at any point during your module, please talk to your Module Tutor. For help and support with your module, visit the ‘Module Contacts’ section of your Canvas course (our Virtual Learning Environment) or speak to your Personal Academic Tutor. 1. Welcome and introduction 2. Module aims and outcomes 3. Module organisation of work and topics 4. Learning and teaching on this module 5. Module Assessments 6. Developing good academic practice 7. Extenuating circumstances and extension 8. How this module is reviewed Welcome and introduction Module aims and outcomes The individual ßnal year project aims to consolidate your learning in previous stages by expanding your knowledge of a relevant topic area which has been covered through the course of your study. By employing research strategies and undertaking focused inquiry on a chosen topic area which relates to working with children, young people and families, you will develop both your knowledge base and the skills needed to gather and present relevant information in a critically reàective way. Module learning outcomes (LOs) 01. Demonstrate in depth knowledge and a systematic, academically informed understanding of a selected topic relevant to your degree. critical understanding of key aspects of theory/practice relating to work with children, young people and families. 02. Apply methods and techniques to review, consolidate, extend and apply your knowledge and understanding to complete a project. Contextualise in-depth knowledge within current academic debates. 03. Identify access and critically evaluate/review the relevant data and literature in systematic manner. 04. Develop your time management and independent problem-solving skills.

examining the over prevalence of Asian community within mental health services. What are the services and specialist interventions

Assignment criteria: Summative Coursework: Final Project Submission (8000words) SEM 2 100%

The actual assessed piece of work for this module is made up of an 8000 word written assignment, which will consolidate your learning in previous stages by expanding your knowledge of a relevant topic area which is related to working with children, young people and families. The structure of the final year project should correspond with the headings provided in your summary

Question of the whole dissertation:

examining the over prevalence of Asian community within mental health services. What are the services and specialist interventions etc.

Chapter 1: 2,000 words

History of mental health legislations talking about the legislations and how mental health legislations have changed so comparing from medieval times to todays world

Consider the legal context. Legislation relating to mental health dates back to medieval times.

• 1601 Poor Relief Act
• 1834 Poor law Amendment Act
• 1845 Lunatic Asylums Act and pauper Lunatics Act
• 1913 mental deficiency Act
• Mental Treatment Act
• 1959 Mental health Act
• !983 Mental health Act
• 2007 Mental health Amendment Act

consider how language has changed. Note that the MHA 1983 is still the main piece of legislation and the 2007 Act only made amendments.

The main thing is spoken about the move that came in the post war years and the 1959 act to provide more care in the community rather than the large asylums. More of a social model approach rather than opposed on medical model. Consider these models and approaches in context of changing cultures. Migration and and changing society and what cause mental health problems.

MOVE ON TO THEN – Talking about how the approaches towards mental health have changed from medical model to social models.

Talk about western model, what the medical model is when was it around and how it was used in mental health, mention DSM and icd10 (Incarceration to decarceration)

And then how that changed to social model, culturally sensitive, UK now has a diverse society today comparing to centuries ago can pick a specific century in which UK had fewer ethnic e.g., BAME AND Asian population living in uk (Add percentages etc.. )

Lok at how treatment and interventions changed so psychiatric medication still prevalent but more humanist approaches such as counselling and CBT approaches. Bring it all up to date in discussing the westernisation pf the BAME community. Related pressures such as social media consider the community as a whole but also talk about women as well.

Chapter two

Is completed

Throughout chapter 2 I have talked about MENTAL HEALTH SERVICES IN SURROUNDING AREAS OF THE UK THAT SUPPORT THE ASIAN COMMUNITIES.

So I found few organisations for instance rethink mental illness, Pakistani resource centre, Roshni, Roshni ghar these charity based mental health services. So I had to talk about what the service is and what their objectives are and what services and support do they provide for Asian communities.

CHAPTER 3: 1, 686 words

Making sure there are title and subtitles placed where appropriately needed not too many tho

1. Main focus of this chapter is: talking the experience of the Asian communities in the mental health system

There views on mental health system and experiences, this will need to be longer than the other question below as this is the focus but do make sure the one below is a good piece that states how mainstream services aren’t as culturally sensitive

2. What professionals in mental health system think is preventing Asian community from accessing services stating for instance

Although government policy mandated that all mental health practitioners develop their “cultural competence” through dedicated training in race equality [2], this has not yet led to marked changes in clinical practice and has been suggested to reproduce institutional racism by continuing to “other” marginalised communities [32].

Where mainstream services have failed to engage South Asian service users, voluntary and community-based organisations have shown to be more effective, due to higher levels of trust, personalised support and being located in the communities they serve [76, 79].

https://link.springer.com/article/10.1007/s40615-021-00993-x#ref-CR77

write a letter to your self 7- 14 months ago.

Description

For this assignment you will have to write a letter to your self 7- 14 months ago. Do you remember how excited you were the day you signed up for this class? Write a motivational letter, let yourself know what happened during the next year or so. Notes on self: Lost job November 2021 Contacted covid, March 2022 Lost my best friend who was 35, February 2022 Had to sleep in my car in a strange state while I left my kids home with a sitter to make ends meat and pay for school, while I fell behind on rent and bills. and do school off of public work while there ( October) 2021 to January 2022) Almost lost my left middle finger off December 2021. Had to have surgery to put it back together. February 2022 obtained another job, finishing nursing school in April, catching up on bills. Getting back on track. Had to keep going as it is/ was my own way out to finish this program at all cost to help me better care for my kids as a solo single mom. My motivation was god and change of life for my kids

Critically Compare Marxist and Functionalist theories of socialisation