chemistry pre lab questions / Enzyme-Catalyzed Oxidation of Tyrosine

1. [4] Inhibitors, I, are compounds that decrease the rate of enzyme catalysed reactions by adding to the mechanism a step
Show how the rate law for the reaction is modified when allowance is made for the participation of an inhibitor in the mechanism proposed by eq.1.
2. [1] In the “Determination of Optimal Wavelength” portion of the experiment, what features are you
looking form in the absorption spectrum. How are the spectral features evolving over time? Look up
relevant extinction coefficients.
3. [2] In the reaction kinetics portion of this experiment, how important is it for the addition of enzyme
to be completed simultaneously for all 6 samples?
4. [2] What is the general shape of the curves that will be obtained from the reaction kinetics portion
of the experiment? Sketch and explain.
5. [1] Based upon the theory presented in the previous pages, what would be the significance of
degassing the solvent before beginning the experiment