Practitioner Research Proposal Essay

Passage Analysis test on Animal Farm By: George Orwell

English

Passage Analysis test on Animal Farm By: George Orwell 

_________/40

Quotation Analysis Test – Animal Farm by George Orwell

Indicate why the following quotes would be identified as important to the story.  Consider any major themes or concepts that relate and be sure to include a character  (10 marks x 4 = 40 marks AP)

1. “Our Leader, Comrade Napoleon, (…) has stated categorically – categorically, comrade – that Snowball was Jones’s agent from the very beginning – yes, and long before the Rebellion was ever thought of.”                                                    ____/10

IMPORTANCE:

2. “If you have your lower animals to contend with…We have our lower classes.”

   ___/10

IMPORTANCE:

3. “Clover looked down at the hillside, her eyes full of tears (…). These scenes of terror and slaughter were not what they had looked forward to on that night when Old Major first stirred them to rebellion.”                                                                    ____/10

IMPORTANCE:

4. “The creatures outside looked from pig to man, and from man to pig, and from pig to man again: but already it was impossible to say which was which.” ____/10

IMPORTANCE:

Why should we care about smoking?

What is the effect of the demyelization on the nerves of an individual who has Multiple Sclerosis?

Psychology

Week 5: Discussion*

YOU MUST COMPLETE THIS TO PASS THIS CLASS.

Answer the following questions, using the case study below:

Neurologic System: Multiple Sclerosis

Discussion Assignment:

• What is the effect of the demyelization on the nerves of an individual who has Multiple Sclerosis?
• Why are the medications appropriate?
• Why do you think females are at greater risk for MS?

Case Study:

Patient: Myrtle, Newly Diagnosed, (25 years old)

Social History:   Homeless, Unemployed, Single,

Nonsmoker; no ETOH (ETOH is an acronym for ethyl alcohol); no illicit drugs

On line college student-struggles to understand material

Symptoms

2006: blurred & double vision

2013: Double vision; urinary urgency; poor attention

2012: Numbness in both legs

Diagnosis: RRMS – Relapse-Remitting Multiple Sclerosis

• • • (2 episodes of neurological symptoms referable to the CNS, separated in space and in time)
    • Memory problems past 6 months; fatigue; heat intolerance; problems with balance
    • Prevalence by Cognitive Domains
    • Memory 40%
    • Information Processing 35%
    • Attention/concentration 30%
    • Problem Solving 20%
    • Visuospastial Abilities 20%
    • Verbal fluency 10%
    • Multiple domains (average) 22%

Supporting the Diagnosis:

• • • Findings on exam:
      • left INO internuclear ophthalmoplegia abbreviation: INO Loss of the normal paired movements of the eyes when tracking an object to the left or right. An INO is marked by the failure of one eye, e.g., the left, to cross the midline during an attempt to see an object on the opposite side of the body, e.g., the right.
      • vertical nystagmus (a repetitive, involuntary, to-and-fro oscillation of the eyes. It may be physiological or pathological and may be congenital or acquired.),
      • right ptosis,
      • MRI Brain and T spine:
        • Multiple enhancing white matter lesions
        • High T2 signal in the right optic nerve
        • Rim enhancing lesion at T6-7CSF
        • Bands notes in CSF and not in serum

Medications

• IM Interferon beta-1a
• Gabapentin 300mg tid
• Oxybutynin Chloride 5mg tid

demonstrate how to use a source within a text. Select an article of your choice and read through it a few times.

“The United Kingdom (UK) has left the European Union (EU) and will once again be free to make its own laws, post the Brexit Transition period. Some argue that the UK’s parliamentary sovereignty was indeed undermined by the EU and that the UK has finally got back its independence.” Discuss this statement.

GFP Protein purification from recombinant vector in E coli cell

GFP Purification from recombinant pQE30 vector inside E.Coli Cell
The abstract
It should be descriptive and specific, essentially “my topic in a nutshell”.
Should be understood separately from the report itself and covers the following: What was done?
Why was it done? How was it done? What was found? What is the significance of the findings?
Introduction
The introduction should present appropriate background material, supported by references. It
should include a statement regarding the major aim/objective(s) of this report.
Experimental Procedures
This report does not warrant a separate section for Materials – describe reagents and apparatus if
needed within the text.
The experimental procedures (Methods) should include all the five major lab modules that were
carried out this semester (you may choose to combine content as you see fit if it is cohesive) as
well as incorporate the methods related to the additional data you have been provided.
Most methods sections are organized under subheadings. If you do use subheadings, make them
functional (e.g., Subcloning the GFP Open Reading Frame) rather than chronological (e.g., Prac 2).
This section should be written in past-tense format and in a concise style to reflect the key
experimental information needed to understand what was done. Use references to key original
citations (e.g., Inoue method for preparing competent cells, Promega Wizard kits for DNA clean up
or minipreps, etc.) but do not reference the practical manual.
Method
1. Cloning of GFP
• PCR amplification GFP open reading frame • Analysis by DNA agarose gel electrophoresis •
Restriction enzyme digestion
2. Restriction enzyme digestions and ligations
• Purification and quantification of digested DNA • Ligation reactions
3. Competent cells and transformation
• Preparation of competent E. coli cells
• Transformation of DNA construct into E. coli cells
4. Cloning confirmation and GFP induction
• Confirmation of clones visually
• Preparation of plasmid DNA from transformed colonies
• Analysis by restriction enzyme digestion and agarose gel electrophoresis
5. Expression and purification of recombinant GFP
• Protein induction and expression in bacteria
• Purification using immobilised metal affinity chromatography
• Analysis by SDS-PAGE
Experimental workflow of GFP Purification from recombinant vector in E.coli.
1.Cloning
Amplification of GFP insert by PCR
Ligation of insert into plasmid vector
Propagation of plasmid
2.Protein expression. Purification of plasmid
Induction of GFP protein expression in bacteria. Restriction analysis
Protein Purification

3.Verification
Verification by SDS-PAGE
Verification by Western blotting
Discussion
In this section, the data should be related back to the literature, ideas presented in the
Introduction and the original aims.
The Discussion should demonstrate a clear understanding of the work through critical analysis and
interpretation of the experimental outcomes and implications. This could include the following
(among others): comparison between observed and expected data – e.g. why was Vent DNA
polymerase used, analysis of PCR product size, ligation reactions, transformation outcomes,
restriction digest analysis to screen for positive colonies); how control reactions were used or
could have been incorporated (e.g. for the PCR amplification of the GFP ORF); how qualitative data
(i.e. SDS-PAGE results) can be enhanced by quantitative protein purification (based on the protein
assays and fold-purification exercise) and qualitative (e.g. Western blot) data acquisition. Any
aberrant data is pointed out and explained logically (if applicable).
You must discuss the additional data provided. Can you probe this data further and rationalize in
the context of your other data? You should discuss the future directions of this work and how
further purification could be achieved. Include at least one modification or addition to the
protocol based on your results which could be incorporated post purification by IMAC and at least
one modification or addition to the protocol which could be incorporated pre purification by
IMAC.

Securing networks (internet, LANs, wireless)

Business problem

Using a Web browser, perform some research on a newer malware variant that has been reported by a major malware containment vendor.

Using a Web browser, perform some research on a newer malware variant that has been reported by a major malware containment vendor. Using a search engine, go to the vendor’s Web site; this could be Symantec, McAfee, or any of their competitors. Visit one malware prevention software vendor. Search for the newest malware variants and pick one. Note its name and try to understand how it works. Now look for information about that same malware from at least one other vendor. Were you able to see this malware at both vendors? If so, are there any differences in how they are reported between the two vendors?